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PMID: 2115243

As for the role that caffeine has in the purported benefits of the coffee enema, drinking coffee is a far more effective method for increasing the amount of caffeine in your blood (and hence causing vasodilation to potentially send toxins from the blood into the colon, or causing increased bile acid secretion to potentially facilitate liver detoxification) compared to administering it at the other end

Nevertheless, the precise molecular mechanisms by which probiotics contribute to colorectal cancer prevention and therapy remain insufficiently elucidated (244)

C[ontrol]), timing (pre-therapy and post-therapy), as well as the interaction of treatment group and timing